Database : HANSEN
Search on : ANTICORPOS ANTIBACTERIAS/SANGUE [Subject descriptor]
References found : 32 [refine]
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Id:13935
Author:Jayapal, V; Selvibai, G; Mahalakshmi, K; Pushkala; Regunath, K; Subramanian, S
Title:Comparative study of anti-PGL-1, anti-35 kDa and anti-lipoarabinomannan assays for serodiagnosis of leprosy ..-
Source:s.l; s.n; 2001. 9 p. tab.
Abstract:Three antibody assays (anti-PGL-1, anti-35 kDa and anti-LAM) were used to determine the levels of antibodies in the sera of untreated leprosy patients. All the three assays showed higher levels of antibodies in BL/LL patients as compared to I and TT/BT patients, as well as healthy controls. BL/LL patients showed positivity of 100 per cent, 84.2 per cent and 78.9 per cent by anti-PGL-1, anti-35 kDa and anti-LAM assays respectively. All the three assays were negative for leprosy in healthy controls. Anti-PGL-1 assay was positive in 20 per cent of TT/BT patients and 17.9 per cent of I patients. Anti-35 kDa assay was negative in all the TT/BT patients and positive in 7.14 per cent of I patients. Anti-LAM assay was positive in 13.3 per cent of TT/BT patients and in 10.7 per cent of I patients. Hence, while these assays are valuable in diagnosing BL/LL patients, their usefulness in diagnosing I, BT or TT leprosy is limited. (AU).
Descriptors:MYCOBACTERIUM LEPRAE/imunol
TESTES SOROLOGICOS
ANTICORPOS ANTIBACTERIAS/sangue
ANTIGENOS DE BACTERIAS/imunol
GLICOLIPIDIOS/imunol
HANSENIASE/sangue
HANSENIASE/clas
HANSENIASE/diag
HANSENIASE/imunol
LIPOPOLISSACARIDIOS/imunol
Limits:ESTUDO COMPARATIVO
HUMANO
MASCULINO
FEMININO
ADULTO
MEIA-IDADE
ADOLESCENTE
Electronic Medium:http://www.ilsl.br
Location:BR191.1; 09162/s


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Id:13719
Author:Kang, T. -J; Kim, S. -K; Lee, S. -B; Chae, G. -T; Kim, J. -P
Title:Comparison of two different PCR amplification products (the 18-kDa protein gene vs. RLEP repetitive sequence) in the diagnosis of Mycobacterium leprae ..-
Source:s.l; s.n; 2003. 5 p. tab.
Abstract:To determine the best molecular method for diagnosing leprosy, two sets of Mycobacterium leprae-specific primers were compared. Fresh biopsies and slit skin smear samples were obtained from 67 leprosy patients and examined by touchdown (TD) PCR using primers amplifying either a 129-bp fragment of the RLEP repetitive sequence or a 360-bp fragment of the 18-kDa protein gene of M. leprae. Seventeen of 30 (56.7 per cent) biopsy specimens and four of 37 (10.8 per cent) slit skin smear specimens were positive using the primer for the 18-kDa protein gene, whereas 24 of 30 (80 per cent) biopsy and 27 of 37 (73 per cent) slit skin smear samples showed detectable PCR products in the RLEP repetitive sequence. Twenty-one of 31 cases (67.7 per cent) with a bacterial index of zero were PCR positive for the primer RLEP repetitive sequence. These results demonstrate that detection of M. leprae using PCR with primers to a RLEP sequence is more sensitive and specific than PCR with the 18-kDa protein gene primers and also slit smears with acid fast staining. PCR of RLEP repetitive sequences is therefore a useful means of detecting M. leprae DNA even when it is present at very low levels. (AU).
Descriptors:ANTICORPOS ANTIBACTERIAS/sangue
PROTEINAS DE BACTERIAS/genet
HANSENIASE/diag
HANSENIASE/microbiol
MYCOBACTERIUM LEPRAE/genet
MYCOBACTERIUM LEPRAE/isol
REACAO EM CADEIA DA POLIMERASE/métodos
PELE/microbiol
SEQUÊNCIAS REPETITIVAS DO ACIDO NUCLEICO
 PRIMERS DO DNA
Limits:ESTUDO COMPARATIVO
HUMANO
Electronic Medium:http://www.ilsl.br
Location:BR191.1; 09117/s


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Id:13639
Author:Kang, T. -J; Kim, S. -K; Lee, S. -B; Chae, G. -T; Kim, J. -P
Title:Comparison of two different PCR amplification products (the 18-kDa protein gene vs. RLEP repetitive sequence) in the diagnosis of Mycobacterium leprae ..-
Source:s.l; s.n; 2003. 5 p. ilus, tab.
Abstract:To determine the best molecular method for diagnosing leprosy, two sets of Mycobacterium leprae-specific primers were compared. Fresh biopsies and slit skin smear samples were obtained from 67 leprosy patients and examined by touchdown (TD) PCR using primers amplifying either a 129-bp fragment of the RLEP repetitive sequence or a 360-bp fragment of the 18-kDa protein gene of M. leprae. Seventeen of 30 (56.7 per cent) biopsy specimens and four of 37 (10.8 per cent) slit skin smear specimens were positive using the primer for the 18-kDa protein gene, whereas 24 of 30 (80 per cent) biopsy and 27 of 37 (73 per cent) slit skin smear samples showed detectable PCR products in the RLEP repetitive sequence. Twenty-one of 31 cases (67.7 per cent) with a bacterial index of zero were PCR positive for the primer RLEP repetitive sequence. These results demonstrate that detection of M. leprae using PCR with primers to a RLEP sequence is more sensitive and specific than PCR with the 18-kDa protein gene primers and also slit smears with acid fast staining. PCR of RLEP repetitive sequences is therefore a useful means of detecting M. leprae DNA even when it is present at very low levels. (AU).
Descriptors:ANTICORPOS ANTIBACTERIAS/sangue
PROTEINAS DE BACTERIAS/genet
PRIMERS DO DNA
HANSENIASE/diag
HANSENIASE/microbiol
MYCOBACTERIUM LEPRAE/genet
MYCOBACTERIUM LEPRAE/isol
REACAO EM CADEIA DA POLIMERASE/métodos
SEQUÊNCIAS REPETITIVAS DO ACIDO NUCLEICO
PELE/microbiol
Limits:ESTUDO COMPARATIVO
HUMANO
Electronic Medium:http://www.ilsl.br
Location:BR191.1; 09054/s


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Id:13625
Author:Antunes, Abílio; Nina, Jaime; David, Suzana
Title:Serological screening for tuberculosis in the community: an evaluation of the Mycodot procedure in an African population with high HIV-2 prevalence (Republic of Guinea-Bissau) ..-
Source:s.l; s.n; 2002. 5 p. tab.
Abstract:The immuno-dot-blot assay MycoDot, which detects lipoarabinomannan (LAM) antibodies, was evaluated for the serological diagnosis of active pulmonary tuberculosis in patients in a rural community in the Republic of Guinea-Bissau. Sera from 269 adults (age > 15) and 33 children (age < 5) were assayed for antibodies in a blind manner and the results compared to the clinical status of tuberculosis. The assay had a specificity and a sensitivity of 92.4 per cent and 63.0 per cent respectively, when applied to the adult population. In HIV-2 infected individuals (27/269), the specificity and sensitivity of the assay were similar, 94.7 per cent and 62.5 per cent respectively. The assay did not provide high sensitivity for the diagnosis of tuberculosis in children. Sera from patients with leprosy cross-reacted with the antigen of the assay. It is concluded that this easily performed assay may be useful for the presumptive diagnosis of tuberculosis in adult populations in rural areas of developing countries where routine screening is not readily available. (AU).
Descriptors:ANTICORPOS ANTIBACTERIAS/sangue
GUINE BISSAU
INFECCOES POR HIV/compl
HIV-2
IMMUNOBLOTTING/métodos
LIPOPOLISSACARIDIOS/uso diag
POPULACAO RURAL
MYCOBACTERIUM TUBERCULOSIS/isol
SENSIBILIDADE E ESPECIFICIDADE
TUBERCULOSE/compl
TUBERCULOSE/diag
TUBERCULOSE/patol
TUBERCULOSE/virol
Limits:ESTUDO COMPARATIVO
HUMANO
PRÉ-ESCOLAR
CRIANÇA
ADULTO
SUPPORT, NON-U.S. GOV'T
ADOLESCENTE
Electronic Medium:http://www.ilsl.br
Location:BR191.1; 09016/s


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Id:11617
Author:Tovar-Rivera, T; Sánchez-Colón, S; Padierna_olivos, L; Massó-Rojas, F; Estrada-Parra, S; Mondragón-Gonzáles, R; Jiménez-Martínez, M. C; Sánchez_garcía, F. J
Title:Connectivity patterns in tuberculosis and leprosy patients are indistinguishable from that of healthy donors ..-
Source:s.l; s.n; 2001. 8 p. graf.
Descriptors:ANTICORPOS ANTIBACTÉRIAS
ANTICORPOS ANTIBACTÉRIAS
ESPECIFICIDADE DE ANTICORPOS
ANTIGENOS DE BACTÉRIAS
IMUNOGLOBULINAS FAB
ENFOQUE ISOELÉTRICO
HANSENIASE
MYCOBACTERIUM LEPRAE
MYCOBACTERIUM TUBERCULOSIS
TUBERCULOSE PULMONAR
Imunoglobulina G/BI
Imunoglobulinas Fab/IM
Location:BR191.1; 08440/s


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Id:11304
Author:Kampirapap, Kowit
Title:Mycobacterium leprae particle agglutination in diagnosis and monitoring of treatment of leprosy ..-
Source:s.l; s.n; 1999. 5 p. tab, graf.
Descriptors:ADOLESCENCIA
TESTES DE AGLUTINAÇAO
TESTES DE AGLUTINAÇAO
ANALISE DE VARIANCIA
ANTICORPOS ANTIBACTÉRIAS
ANTIGENOS DE BACTÉRIAS
CRIANÇA
PRÉ-ESCOLAR
MONITORAMENTO DE MEDICAMENTOS
MONITORAMENTO DE MEDICAMENTOS
GLICOLIPIDIOS
LEPROSTATICOS
HANSENIASE
HANSENIASE
MYCOBACTERIUM LEPRAE
SENSIBILIDADE E ESPECIFICIDADE
Location:BR191.1; 07243/s


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Id:11296
Author:Rojas, R. E; Demichelis, S. O; Sarno, E. N; Segal-Eiras, A
Title:IgM anti-phenolic glycolipid I and IgG anti 10-kDa heat shock protein antibodies in sera and immune complexes isolated from leprosy patients with or without erythema nodosum leprosum and contacts ..-
Source:s.l; s.n; 1997. 10 p. tab, graf.
Descriptors:ADOLESCENCIA
ANTICORPOS ANTIBACTÉRIAS
ANTICORPOS ANTIBACTÉRIAS
ANTICORPOS ANTIBACTÉRIAS
ANTICORPOS MONOCLONAIS
COMPLEXO ANTIGENO-ANTICORPO
COMPLEXO ANTIGENO-ANTICORPO
COMPLEXO ANTIGENO-ANTICORPO
ANTIGENOS DE BACTÉRIAS
CHAPERONINA 10
CHAPERONINA 10
ELISA
ERITEMA NODOSO
ERITEMA NODOSO
GLICOLIPIDIOS
IGG
IGM
HANSENIASE LEPROMATOSA
HANSENIASE LEPROMATOSA
POLIETILENOGLICOIS
PROTEINAS RECOMBINANTES
Limits:ESTUDO COMPARATIVO
Location:BR191.1; 07235/s


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Id:11295
Author:Buhrer-Sekula, Samira; Cunha, Maria da Graca Souza; Ferreira, Willian Antunes; Klatser, Paul R
Title:The use of whole blood in a dipstick assay for detection of antibodies to mycobacterium leprae: a field evaluation ..-
Source:s.l; s.n; 1998. 5 p. tab, graf.
Descriptors:ANTICORPOS ANTIBACTÉRIAS
SANGUE
ELISA
ESTUDOS DE AVALIAÇAO
GLICOLIPIDIOS
HANSENIASE
MYCOBACTERIUM LEPRAE
SENSIBILIDADE E ESPECIFICIDADE
TESTES SOROLOGICOS/*MT
Limits:ESTUDO COMPARATIVO
Location:BR191.1; 07234/s


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Id:11022
Author:Triccas, James A; Roche, Paul W; Britton, Warwick J
Title:Specific serological diagnosis of leprosy with a recombinant mycobacterium leprae protein purified from a rapidly growing mycobacterial host ..-
Source:s.l; s.n; aug. 1998. 3 p. tab, graf.
Descriptors:ANTICORPOS ANTIBACTÉRIAS
ANTIGENOS DE BACTÉRIAS
ANTIGENOS DE BACTÉRIAS
ELISA
HANSENIASE
MYCOBACTERIUM LEPRAE
PROTEINAS RECOMBINANTES
SENSIBILIDADE E ESPECIFICIDADE
Location:BR191.1; 07279/s


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Id:11017
Author:Partida-Sanchez, S; Favila-Castillo, L; Pedraza-Sanchez, S; Gomez-Melgar, M; Saul, A; Estrada-Parra, S; Estrada-Garcia, I
Title:IgG antibody subclasses, tumor necrosis factor and IFN-gamma levels in patients with type II lepra reaction in thalidomide treatment ..-
Source:s.l; s.n; 1998. 7 p. tab, graf.
Descriptors:ADOLESCENCIA
ANTICORPOS ANTIBACTÉRIAS
ANTICORPOS ANTIBACTÉRIAS
INTERPRETAÇAO ESTATISTICA DE DADOS
ERITEMA NODOSO
INTERFERON TIPO II
INTERFERON TIPO II
LEPROSTATICOS
LEPROSTATICOS
LEPROSTATICOS
HANSENIASE DIMORFA
HANSENIASE DIMORFA
HANSENIASE TUBERCULOIDE
HANSENIASE TUBERCULOIDE
MYCOBACTERIUM LEPRAE
TALIDOMIDA
TALIDOMIDA
TALIDOMIDA
FATOR DE NECROSE TUMORAL/ME
IMUNOGLOBULINA M/BL/CL
FATOR DE NECROSE TUMORAL/*ME
Limits:ESTUDO COMPARATIVO
Location:BR191.1; 07285/s


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Id:10965
Author:El-Zaatari, Fouad A. K; Naser, Saleh A; Hulten, Kristina; Burch, Paula; Graham, David Y
Title:Characterization of mycobacterium paratuberculosis p36 antigen and its seroreactivities in Crohn's diseases ..-
Source:s.l; s.n; 1999. 5 p. ilus, tab.
Descriptors:HANSENIASE
MYCOBACTERIUM PARATUBERCULOSIS
ANTICORPOS ANTIBACTÉRIAS
ANTIGENOS DE BACTÉRIAS
ANTIGENOS DE BACTÉRIAS
ANTIGENOS DE BACTÉRIAS
DOENÇA DE CROHN
DNA BACTERIANO
ELETROFORESE EM GEL DE POLIACRILAMIDA
IMMUNOBLOTTING
PROTEINAS RECOMBINANTES
ANALISE DE SEQUENCIA DE DNA
TUBERCULOSE
Location:BR191.1; 07428/s


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Id:10309
Author:Barral-Netto, M; Santos, S; Santos, I; von Sohsten, R; Bittencourt, A. L; Carvalho, E. M; Barral, A; Waters, M
Title:Immunochemotherapy with interferon-y and multidrug therapy for multibacillary leprosy ..-
Source:s.l; s.n; 1999. 7 p. tab, graf.
Descriptors:ADOLESCENCIA
ANTICORPOS ANTIBACTÉRIAS
CRIANÇA
CLOFAZIMINA
DAPSONA
QUIMIOTERAPIA COMBINADA
INTERFERON TIPO II
LEPROSTATICOS
HANSENIASE
HANSENIASE
TRANSFORMAÇAO LINFOCITICA
RIFAMPINA
PELE
INTERFERON GAMA RECOMBINANTE/*TU
NEOPTERINA/AN
Location:BR191.1; 07414/s


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Id:10280
Author:Verhagen, Claudia; Faber, William; Klatser, Paul; Buffing, Anita; Naafs, Ben; Das, Pranab
Title:Immunological analysis of in situ expression of mycobacterial antigens in skin lesions of leprosy patients across the histopathological spectrum: association of mycobacterial lipoarabinomannan (LAM) and Mycobacterium leprae phenolic glycolipid-I (PGL-I) with leprosy reactions ..-
Source:s.l; s.n; 1999. 12 p. ilus, tab.
Descriptors:ANTICORPOS ANTIBACTÉRIAS
ANTICORPOS MONOCLONAIS
ESPECIFICIDADE DE ANTICORPOS
ANTIGENOS DE BACTÉRIAS
ANTIGENOS DE BACTÉRIAS
CHAPERONINAS
CHAPERONINAS
GLICOLIPIDIOS
GLICOLIPIDIOS
IMUNOHISTOQUIMICA
HANSENIASE
HANSENIASE
HANSENIASE
LIPOPOLISSACARIDIOS
LIPOPOLISSACARIDIOS
MACROFAGOS
MYCOBACTERIUM LEPRAE
MYCOBACTERIUM LEPRAE
MYCOBACTERIUM LEPRAE
VALOR PREDITIVO DOS TESTES
ESTUDOS RETROSPECTIVOS
DERMATOPATIAS
DERMATOPATIAS
DERMATOPATIAS
Location:BR191.1; 07419/s


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Id:10215
Author:Singh, Satish; Jenner, Peter J; Narayan, N. P. Shanker; Ramu, Gopal; Colston, M. Joseph; Prasad, H. Krishna; Nath, Indira
Title:Critical residues of the mycobacterium leprae LSR recombinant protein discriminate clinical activity in erythema nodosum leprosum reactions ..-
Source:s.l; s.n; 1994. 4 p. tab, graf.
Descriptors:SEQUENCIA DE AMINOACIDOS
ANTICORPOS ANTIBACTÉRIAS
ANTIGENOS DE BACTÉRIAS
LINFOCITOS B
PROTEINAS DE BACTÉRIAS
ESTUDOS DE CASOS E CONTROLES
MAPEAMENTO DE EPITOPOS
EPITOPOS
ERITEMA NODOSO
HANSENIASE LEPROMATOSA
DADOS DE SEQUENCIA MOLECULAR
MYCOBACTERIUM LEPRAE
FRAGMENTOS DE PEPTIDIOS
PROTEINAS RECOMBINANTES DE FUSAO
Location:BR191.1; 06916/s


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Id:10029
Author:Buhrer, Samira S; Smits, Henk L; Gussenhoven, George C; Van Ingen, Cor W; Klatser, Paul R
Title:A simple dipstick assay for the detection of antibodies to phenolic glycolipid-I of Mycobacterium leprae ..-
Source:s.l; s.n; 1998. 4 p. tab.
Descriptors:HANSENIASE
HANSENIASE
HANSENIASE
ANTICORPOS ANTIBACTÉRIAS
ANTIGENOS DE BACTÉRIAS
ELISA
GLICOLIPIDIOS
IGM
LUZ
MYCOBACTERIUM LEPRAE
PRESERVAÇAO BIOLOGICA
FITAS REAGENTES
REPRODUTIBILIDADE DE RESULTADOS
FATORES DE TEMPO
Location:BR191.1; 06994/s


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Id:9942
Author:Hatta, Mochammed; Izumi, Shinzo; Klatser, Paul R
Title:Evaluation of the Mycobacterium leprae particle agglutination (MLPA) test as a tool in the epidemiology of leprosy in high prevalence village in South Sulawesi, Indonesia ..-
Source:s.l; s.n; 1995. 5 p. tab.
Descriptors:HANSENIASE
HANSENIASE
HANSENIASE
ANTICORPOS ANTIBACTÉRIAS
IGM
TRIAGEM DE MASSA
MYCOBACTERIUM LEPRAE
PREVALENCIA
ESTUDOS PROSPECTIVOS
SAUDE SUBURBANA
Location:BR191.1; 06849/s


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Id:9935
Author:Baelden, M. C; Bouckaert, A. E; Gregoire, D; Van de Poele, M; Coene, M
Title:Comparison of thermostable macromolecular antigens from leprosy-associated bacteria ..-
Source:s.l; s.n; 1994. 5 p. ilus, tab.
Descriptors:HANSENIASE
MYCOBACTERIUM
MYCOBACTERIUM BOVIS
MYCOBACTERIUM LEPRAE
ANTICORPOS ANTIBACTÉRIAS
ANTIGENOS DE BACTÉRIAS
CORYNEBACTERIUM
REAÇOES CRUZADAS
ELISA
IMUNOELETROFORESE
ESPECIFICIDADE DE ESPÉCIES
Location:BR191.1; 06856/s


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Id:9893
Author:Hatta, Mochammad; Van Beers, Stella M; Madjid, Baedah; Djumadi, Achmad; de Wit, Madeleine Y. L; Klatser, R
Title:Distribution and persistence of Mycobacterium leprae nasal carriage among a population in which leprosy is endemic in Indonesia ..-
Source:s.l; s.n; 1995. 5 p. ilus, tab.
Descriptors:ADOLESCENCIA
ANTICORPOS ANTIBACTÉRIAS
PORTADOR
PORTADOR
CRIANÇA
CRECHES
PRÉ-ESCOLAR
ANALISE POR CONGLOMERADOS
SEGUIMENTOS
INDONÉSIA
HANSENIASE
HANSENIASE
HANSENIASE
MYCOBACTERIUM LEPRAE
NARIZ
REAÇAO EM CADEIA POR POLIMERASE
PREVALENCIA
Location:BR191.1; 06784/s


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Id:9831
Author:Wichitwechkarn, Jesdawan; Karnjan, Somjit; Shuntawuttisettee, Siriphan; Sornprasit, Chaluay; Kampirapap, Kowit; Peerapakorn, Somchai
Title:Detection of Mycobacterium leprae infection by PCR ..-
Source:s.l; s.n; 1995. 5 p. ilus, tab.
Descriptors:HANSENIASE
HANSENIASE
HANSENIASE
ANTICORPOS ANTIBACTÉRIAS
QUIMIOTERAPIA
GLICOLIPIDIOS
REAÇAO EM CADEIA POR POLIMERASE
PELE
RESULTADO DE TRATAMENTO
TAILANDIA
Location:BR191.1; 06838/s


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Id:9716
Author:Sanchez Monton, T; Martin Luengo, F
Title:Serodiagnostico en la tuberculosis pulmonar. Valoracion clinica ..-
Source:s.l; s.n; 1992. 5 p. tab, graf.
Descriptors:ANTICORPOS ANTIBACTÉRIAS
ESPECIFICIDADE DE ANTICORPOS
ANTIGENOS DE BACTÉRIAS
ESTUDOS DE CASOS E CONTROLES
ELISA
ESTUDOS DE AVALIAÇAO
IMUNOGLOBULINAS
IMUNOGLOBULINAS
HANSENIASE
PNEUMOPATIAS
MYCOBACTERIUM TUBERCULOSIS
MYCOBACTERIUM TUBERCULOSIS
VALOR PREDITIVO DOS TESTES
SENSIBILIDADE E ESPECIFICIDADE
ESCARRO
TUBERCULOSE PULMONAR
TUBERCULOSE PULMONAR
TUBERCULOSE PULMONAR
Location:BR191.1; 06514/s



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